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Protein concentration was quantified using the BCA assay and aliquots of 250 g protein were solubilized with digitonin (1:6 digitonin to protein ratio) for 10 min on ice
The study involved data from 16,827 patients evaluated by neuro-ophthalmologists, and focused on patients with type 2 diabetes or those who were overweight or obese, comparing those prescribed semaglutide to those using non-glucagon-like peptide-1 receptor agonist (GLP-1 RA) medications
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